Which instrument monitoring method involves using biological indicators?

Biological monitoring is a critical method used to ensure the effectiveness of sterilization processes in a dental practice. This approach specifically involves the use of biological indicators, which are living organisms that can be used to assess whether sterilization has occurred effectively. Typically, these indicators contain bacterial spores that are highly resistant to sterilization methods. After a sterilization cycle, these indicators are incubated to check for any growth of bacteria. If no growth is observed, it confirms that the sterilization process was successful.

Using biological monitoring is essential as it provides a direct measure of the sterilization process's effectiveness and the capability to kill even the most resistant microorganisms. This method stands out among monitoring techniques as it uses actual organisms to verify the performance of sterilizers, rather than indirect measures like physical changes or chemical reactions. Therefore, biological monitoring is crucial in infection control to ensure the safety and health of both patients and dental practitioners.